Which of the following statements will be correct regarding restriction endonucleases used in constructing recombinant DNA molecules?
1.
They are RNA molecules acting as enzymes.
2.
The enzymes used in such experiments cut dsDNA randomly at any site.
3.
They have been isolated from bacterial cells.
4.
All restriction enzymes are thermostable and hence can be used in PCR procedures.
Subtopic: Restriction Enzymes - Main Enzymes | Restriction Enzymes: Historical Background |
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DNA fragments created by the action of restriction enzymes can be separated by agar gel electrophoresis where:
1. Only I is correct
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
| I: | larger the fragment size, the farther it moves in the gel |
| II: | the DNA fragments are forced to move towards anode under an electric field |
2. Only II is correct
3. Both I and II are correct
4. Both I and II are incorrect
Subtopic: Separation and Isolation of DNA fragments |
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Level 1: 80%+
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A ‘continuous culture system’ method usually produces a larger biomass mainly because:
| 1. | the environmental conditions for the growth of the microbes are very easily maintained in such a system |
| 2. | such a system keeps most of the cells in their active/log exponential phase |
| 3. | it is very cheap with respect to other systems and hence cost of production is low |
| 4. | such a system uses larger sized bioreactors than other systems |
Subtopic: Large Scale Production |
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Level 2: 60%+
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Animal cells cannot be transformed with the help of:
| 1. | Microinjection | 2. | Ti Plasmid |
| 3. | Retroviral vectors | 4. | Liposomes |
Subtopic: Transforming Plant & Animal Cell |
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Work of which of the following scientists was the basis upon which the discipline of biotechnology was founded?
| 1. | Cohen and Boyer |
| 2. | Watson and Crick |
| 3. | Hershey and Chase |
| 4. | Avery, Macleod and McCarty |
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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The DNA sequence shown below is:
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
5’ – GAATTC – 3’
3’ – CTTAAG – 5’
| 1. | the consensus sequence for prokaryotic promoter |
| 2. | the most common VNTR in the human genome |
| 3. | the recognition sequence of a common restriction endonuclease EcoR I |
| 4. | an intron within the eukaryotic split gene |
Subtopic: Restriction Enzymes - Main Enzymes |
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Level 1: 80%+
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Which of the following statements regarding restriction endonucleases used in recombinant DNA procedures is not true?
| 1. | They are isolated from bacteria |
| 2. | They are synthesized by bacteria as a mechanism of defence against bacteriophages |
| 3. | The type II enzymes, commonly used in rDNA experiments, cut dsDNA at specific recognition sequences |
| 4. | The bacterial DNA does not have recognition sequences for the restriction enzymes produced by itself |
Subtopic: Restriction Enzymes - Main Enzymes |
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It is desirable to use restriction enzymes that create sticky ends in recombinant DNA technology procedures because this:
| 1. | facilitates the action of DNA ligase |
| 2. | makes selection of recombinant DNA possible |
| 3. | increases the copy number of rDNA per cell |
| 4. | will create multiple fragments of the vector DNA |
Subtopic: Tools: Enzymes: I | Tools: Enzymes: II | Tools |
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Plasmids are good cloning vectors mainly because:
| 1. | they are circular |
| 2. | they replicate autonomously |
| 3. | they are extra-chromosomal DNA molecules found in many bacterial cells |
| 4. | they carry genes vital for normal survival and reproduction |
Subtopic: Tools: Vectors: I |
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In a plasmid such as pBR322:
| I: | Ori controls the copy number of DNA per cell |
| II: | Multiple cloning sites are recognition sequences of common restriction enzymes |
| III: | Genes for antibiotic resistance are used as selectable markers |
| 1. | Only I and II are correct |
| 2. | Only I and III are correct |
| 3. | Only II and III are correct |
| 4. | I, II, and III are correct |
Subtopic: Cloning Vector |
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Level 3: 35%-60%
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