The plasmid pBR322 does not contain:

1. An origin of replication site

2. A gene that encodes for restrictor of plasmid copy number

3. Gene for Ampicillin and Streptomycin resistance

4. Sites for many restriction enzymes

Concept Questions :-

Tools: Vectors:II
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Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

Concept Questions :-

Tools: Enzymes:II
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The bioreactor shown in the diagram is:

1. Sparged stirred tank type

2. Airlift type

3. Simple stirred tank type

4. Fluidized bed type

Concept Questions :-

Large Scale Production: II
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What is true about the steps of PCR shown in the diagram given below?

l. Step A occurs at the temperature of 95-98o C

ll. Step B occurs at the temperature of 55o C

lll. Step C occurs at the temperature of 72o C

1. l and ll only

2. l and lll only

3. ll and lll only

4. l, ll and lll

Concept Questions :-

Large Scale Production: I
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A and B in the pBR 322, shown in the diagram given below, respectively represent recognition sequences of :

1. BamH I and Sma I

2. Hind II and Sma I

3. BamH I and SaI I

4. SaI I and Hind II

Concept Questions :-

Tools: Vectors:II
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What is true A,B and C in the given diagrammatic representation of rDNA technology ?

I. At A same restriction enzyme is used to cut both foreign and vector DNA

II. The enzyme used at B is DNA ligase

III. Step C can be called as transformation

1. I and II only

2. I and III only

3. II and III only

4. I,II and III

Concept Questions :-

Intro & History: II
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The process of separation and purification of expressed protein before marketing is called

(1) upstream processing

(2) downstream processing

(3) bioprocessing

(4) postproduction processing

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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

(a) The larger the fragment size, the farther it moves

(b) The smaller the fragment size, the farther it moves

(c) Positively charged fragments move to farther end

(d) Negatively charged fragments do not move

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A gene, whose expression helps to identify transformed cells is known as

(a) selectable marker                  (b) vector

(c) plasmid                                 (d) structural gene

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The DNA fragments separated on an agarose gel can be visualised after staining with

(a) bromophenol blue

(b) acetocarmine

(c) aniline blue 

(d) ethidium bromide

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