NEET Zoology Biotechnology Principles and Processes Questions Solved

A cloning vector has two antibiotic resistance genes- for tetracycline and ampicillin. A foreign DNA

was inserted into the tetracycline gene. Non-recombinants would survive on the medium containing :

1. ampicillin but not tetracycline

2. tetracycline but not ampicillin

3. both tetracycline and ampicillin

4. neither tetracycline nor ampicillin

(3) Both genes are functional in non-recombinants. In recombinants the gene for tetracycline resistance would be insertionally inactivated.

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