Q. No.The specific palindromic sequence which is recognized by EcoRI is:
1.
5' -GGAACC-3'
3'-CCTTGG-5'
2.
5'-CTTAAG-3'
3'-GAATTC-5'
3.
5'-GGATCC-3'
3'-CCTAGG-5'
4.
5'-GAATTC-3'
3'-CTTAAG-5'
3'-CCTTGG-5'
3'-GAATTC-5'
3'-CCTAGG-5'
3'-CTTAAG-5'
Identify the wrong statement with regards to restriction enzyme:
| 1. | They cut the strand at palindromic sites. |
| 2. | They are useful in genetic engineering. |
| 3. | Sticky ends can be joined by using DNA ligases. |
| 4. | Each restriction enzyme functions by inspecting the length of a DNA sequence. |
In-gel electrophoresis, separated DNA fragments can be visualized with the help of:
1. Ethidium bromide in UV radiation
2. Acetocarmine in UV radiation
3. Ethidium bromide in infrared radiation
4. Acetocarmine in bright blue light
| 1. | Ori site | 2. | Palindromic sequence |
| 3. | Recognition site | 4. | Selectable marker |
First discovered restriction endonuclease that always cuts DNA molecules at a particular point by recognizing a specific sequence of six base pairs is:
1. EcoRI
2. Adenosine deaminase
3. Thermostable DNA polymerase
4. Hind II
In recombinant DNA technology antibiotics are used:
1. to keep medium bacteria-free
2. to detect alien DNA
3. to impart disease-resistance to the host plant
4. as selectable markers
Match the following techniques or instruments with their usage:
| (a) | Bioreactor | (i) | Separation of DNA fragments |
| (b) | Electrophoresis | (ii) | Production of large quantities of products |
| (c) | PCR | (iii) | Detection of pathogen, based on antigen-antibody reaction |
| (d) | ELISA | (iv) | Amplification of nucleic acids |
Select the correct option from the following:
| 1. | (a)-(iii), (b)-(ii), (c)-(iv), (d)-(i) |
| 2. | (a)-(ii), (b)-(i), (c)-(iv), (d)-(iii) |
| 3. | (a)-(iv), (b)-(iii), (c)-(ii), (d)-(i) |
| 4. | (a)-(ii), (b)-(i), (c)-(iii), (d)-(iv) |
In a mixture, DNA fragments are separated by-
1. Bioprocess engineering
2. Restriction digestion
3. Electrophoresis
4. Polymerase chain reaction
Spooling is:
| 1. | Amplification of DNA |
| 2. | Cutting of separated DNA bands from the agarose gel |
| 3. | Transfer of separated DNA fragments to synthetic membranes |
| 4. | Collection of isolated DNA |
Select the correct statement from the following:
| 1. | Gel electrophoresis is used for the amplification of a DNA segment. |
| 2. | The polymerase enzyme joins the gene of interest and the vector DNA. |
| 3. | Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions. |
| 4. | PCR is used for the isolation and separation of genes of interest. |
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