Q. 57 Name the five key tools for accomplishing the tasks of recombinant DNA technology. Also mention the functions of each tool.
(i) Restriction endonucleases for cutting the desired DNA at desired places.
(ii) Gel electrophoresis for separating the desired DNA fragments.
(iii) Ligase enzyme for creating recombinant DNA molecule.
(iv) DNA delivery system like electroporation, microinjection. gene gun method, etc.
(v) Competant host (usually bacteria/yeast) to take up the recombinant DNA.