Q. 50 You have identified a useful gene in bacteria. Make a flow chart of the steps that you would follow to transfer this gene to a plant.

After identitying a useful gene in bacteria, following steps should be followed

(i) Isolation of useful gene using restriction endonucleases

(ii) Transferring the gene to a suitable vector to create a recombinant DNA molecule

(iii) Transfer of these recombinant DNA molecules to the target cells.

(iv) Screening of cells for transformation

(v) Selection of transformed cells

(vi) Regeneration of plants from the transformed cells to get transgenic plants.