Ncert Exercises & Solutions

Q.33 Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific’-recognition sequence? What would be the disadvantage if they do not cut the DNA at
specific-recognition sequence?

If the restriction enzymes would cut DNA at random sites instead of al specific sites, then the DNA fragments obtained will not have ‘sticky ends’. In the absence of sticky ends, construction of recambinant DNA molecule would not be possible.

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