Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
1. Laboratory flask of largest capacity
2. A stirred-tank bioreactor without in-lets and outlets
3. A continuous culture system
4. Any of the above
The cultures may be used for extracting the desired protein and then purifying it by using different separation techniques.
The cells can also be multiplied in a continuous culture system where in the used medium is drained out from one side while fresh medium is added from the other to maintain the cells in their physiologically most active log/exponential phase. This type of culturing method produces a larger biomass leading to higher yields of desired protein.
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