Q. 13. DNA is a polymer of nucleotides which are linked to each other by 3'-5' phosphodiester bond. To prevent the polymerisation of nucleotides, which of the following modifications would you choose?
(a) Replace purine with pyrimidines
(b) Remove/Replace 3' OH group in deoxyribose
(c) Remove/Replace 2' OH group with some other group in deoxyribose
(d) Both (b) and (c)
Ans. (b). The enzyme called DNA polymerase progressively adds deoxyribonucleotides to the free 3'-end of the growing polynucleotide chain so, that replication of the 3'-5' strand of the DNA molecule is continuous (growth of the new strand in 5'3' direction).
So, to prevent the polymerization of nucleotides 3' OH group in deoxyribose should be replaced/removed.
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