With regard to the biological nitrogen fixation by Rhizobium in association with soyabean, which one of the following statement/ statements does not hold true.

1. Nitrogenase may require oxygen for its functioning.
2. Nitrogenase is Mo-Fe protein
3. Leg-haemoglobin is a pink coloured pigment.
4. Nitrogenase helps to convert N2 gas into two molecules of ammonia.

The enzyme, nitrogenase which is capable of nitrogen reduction is present exclusively in prokaryotes (e.g., Rhizobium). It is highly sensitive to O2 and gets inactivated when exposed to it, thus does not require oxygen for its functioning. Other statements (b), (c) and (d) are true.
Page 203, XI NCERT