Q. 7.  Give an account of the methods used in sequencing the human genome.

 

Sequencing of human genome has made it possible to understand the link between various genes and their functions. If there are any gene defects that express as disorders of that increase the susceptibility of an individual to a disease then specific gene therapies can be worked out

Methodologies of human genome sequencing

The methods involve two major approaches

(i) Expressed Sequence Tags (ESTs) This method focusses on identifying all the genes that are expressed as RNA.

(ii) Sequence annotation It is an approach of simply sequencing the whole set of the genome that contains all the coding and non-coding sequences, and later assigning different regions in the sequence with functions.

For sequencing, first the total DNA from cell is i.e., isolated and broken down in relatively small sizes as fragments.

Three DNA fragments are cloned in suitable host using suitable vectors. When bacteria is used as vector, they are called Bacterial Artificial Chromosomes (BAC) and when yeast is used as vector, they are called Yeast Artificial Chromosomes (YACs).

Frederick Sanger developed a principle according to which the fragments of DNA are sequenced by automated DNA sequences.

On the basis of overlapping regions on DNA fragments, these sequences are arranged accordingly. For alignment of these sequences, specialised computer0based programmes were developed.

Finally, the genetic and physical maps of the genome were constructed by collecting information about certain repetitive DNA sequences and DNA polymorphism, based on endonuclease recognition sites.