Ncert Exercises & Solutions

Q. 11. You are repeating the Hershey-Chase experiment and are provided with two isotopes ${}_{32}P and {}_{15}N$ (in place of ${}_{35}S$ in the original experiment). How does you expect your results to be different?

Use of

{}_{15}N

will be inappropriate because the method of detection of

{}_{32}P and {}_{15}N

different (

{}_{32}P

being a radioactive isotope while

{}_{15}N

is non-radioactive but is the heavier isotope of nitrogen).
Even if

{}_{15}N

was radioactive then its presence would have been detected, both inside the cell (

{}_{15}N

incorporated as a nitrogenous base in DNA) as well as in the supernatant, because

{}_{15}N

would also get incorporated in amino group of amino acids in proteins. Hence, the use of

{}_{15}N

would not give any conclusive results.

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