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#5 | Med Applications & Humulin
(Zoology) > Biotechnology and its Application

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The two polypeptides of human insulin are linked together by

(1) phosphodiester bonds

(2) covalent bonds

(3) disulphide bridges

(4) hydrogen bonds

 

 90%
Level 1: 80%+
NEET - 2016
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Human insulin is being commercially produced from a transgenic species of

1. Escherichia

2. Mycobacterium

3. Rhizobium

4. Saccharomyces

 89%
Level 1: 80%+
NEET - 2008
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Which of the following statements is false?

I. Insulin was originally extracted from pancreas of slaughtered pigs and cattle
II. Animal insulin is difficult to obtain
III. Animal insulin is identical to human insulin
IV. Non-human insulin caused some patients to develop allergy
V. Recombinant DNA allowed scientists to insert a human insulin gene into a bacterial expression vector
VI. Recombinant insulin is actually obtained from E. coli bacterial cell

1. Only I and II

2. Only III and IV

3. Only III

4. Only VI

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Level 2: 60%+
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Which step proved to be the main challenging obstacle in the production of human insulin by genetic engineering? 

1. Splitting A and B polypeptide chains
2. Addition of C-peptide to pro-insulin
3. Getting insulin assembled into mature form
4. Removal of C-peptide from active insulin
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Humulin production through microbial factories does not include the incorporation of the gene for

1. A peptide 2. B peptide
3. C peptide 4. Both (1) and (2)
 87%
Level 1: 80%+
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