VNTR are useful in DNA profiling because they:

I. Are hypervariable

II. Are inherited

III. Synthesize constitutive enzymes

(1) I, II, III are correct

(2) I and II are correct

(3) I and III are correct

(4) II and III are correct   

Consider the following statements:

I. The largest known human gene is dystrophin at 2.4 million bases

II. Repeated sequences make up very large portion of the human genome

III. There are about 2.4 million locations where single base DNA differences occur in humans

Which of the above statements are true?

1. I and II only

2. I and III only

3. II and III only

4. I, II and III

Which of the following is not required for any of the techniques of DNA fingerprinting available at present ?

(1) Zinc finger analysis

(2) Restriction enzymes

(3) DNA-DNA hybridisation

(4) Polymerase chain reaction

Satellite DNA is important because it

1. codes for protiens needed in cell cycle

2. shows high degree of polymorphism in population and also the same degree of

polymorphism in an individual, which is heritable from parents to children

3. does not code for protiens and is same in all members of the population

4. codes for enzymes needed for DNA replication.

PCR and Restriction Fragment Length Polymorphism are the methods for

1. study of enzymes

2. genetic transformation

3. DNA sequencing

4. genetic fingerprinting

Satellite DNA is useful tool in

(1) organ transplantation

(2) sex determination

(3) forensic science

(4) genetic engineering

Which one of the following pairs of terms / names mean one and the same thing?

(1) Gene pool - genome

(2) Codon – gene

(3) Cistron – triplet

(4) DNA fingerprinting - DNA profiling

What is the first step in the Southern Blot technique?

(1) Denaturation of DNA on the gel for hybridization with specific probe

(2) Production of a group of genetically identical cells

(3) Digestion of DNA by restriction enzyme

(4) Isolation of DNA from a nucleated cell such as the one from the scene of crime

Some of the steps of DNA fingerprinting are given below. Identify their correct sequence from the options given.

A. Electrophoresis of DNA fragments

B. Hybridisation with DNA probe

C. Digestion of DNA by restriction endonucleases

D. Autoradiography

E. Blotting of NDA fragments to nitrocellulose membrane

(1) C – A – B – E – D

(2) C – A – E – B – D

(3) A – E – C – B – D

(4) A – C – E – D – B

Which one of the following set meaning is not different?