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Q. No.Assertion(A): Restriction enzymes are molecular scissors.
Reason(R): They identify DNA sequence and cut them.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Selectable Markers |
92%
Level 1: 80%+
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Assertion(A): The first discovered restriction endonuclease was Hind II.
Reason(R): Hind III was discovered earlier than Hind II.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Hind III was discovered earlier than Hind II.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Tools: Vectors: I | Selectable Markers: I | Selectable Markers: II |
86%
Level 1: 80%+
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| Assertion(A): | Palindromic sequences are identified by restriction endonucleases. |
| Reason(R): | Endonucleases cannot destroy the phosphodiester bonds other than those of palindromes. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Selectable Markers: I | Selectable Markers: II |
51%
Level 3: 35%-60%
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Assertion(A): While loading the DNA fragment into the wells of agarose gel, a blue dye methylene blue is mixed.
Reason(R): Radioactive methylene blue is used.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Radioactive methylene blue is used.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Separation and Isolation of DNA fragments |
Level 4: Below 35%
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Assertion(A): In order to link the alien DNA, the vector needs to have very few, preferably single, recognition site for the commoniy used restriction enzymes.
Reason(R): Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Cloning Vector |
91%
Level 1: 80%+
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Assertion(A): Only antibiotic resistant gene in vectors can be used as selectable markers.
Reason(R): p-gal gene cannot be used in plasmid vectors as selectable markers.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): p-gal gene cannot be used in plasmid vectors as selectable markers.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Selection of Recombinant Transformants |
Level 3: 35%-60%
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| Assertion(A): | Foreign DNA can be inserted into host cells by heat shock treatment, microinjection and biolistics. |
| Reason(R): | The chromogenic substrate used in blue-white screening of recombinant DNA is X-gal. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Transforming Plant & Animal Cell | Host & Desired DNA | Large Scale Production |
66%
Level 2: 60%+
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Assertion(A): Alkaline phosphatase is used to prevent unwanted self ligation of vector DNA.
Reason(R): Alkaline phosphatase removes phosphate group at 5' end of DNA molecule.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): Alkaline phosphatase removes phosphate group at 5' end of DNA molecule.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Tools: Enzymes: I | Tools: Vectors: II |
72%
Level 2: 60%+
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Assertion(A): Plasmids are extrachromosomal gentical material of bacteria.
Reason(R): These are genetic material present apart from the nucleoid.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Reason(R): These are genetic material present apart from the nucleoid.
1. Both (A) and (R) are true and (R) is the correct explanation of (A).
2. Both (A) and (R) are true but (R) is not the correct explanation of (A).
3. (A) is true but (R) is false.
4. Both (A) and (R) are false.
Subtopic: Cloning Vector |
70%
Level 2: 60%+
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| Assertion(A): | Plasmids as cloning vectors usually get integrated to the host cells chromosome after transformation. |
| Reason(R): | Plasmids get disintegrated after transformation. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is True but (R) is False. |
| 4. | Both (A) and (R) are False. |
Subtopic: Cloning Vector |
Level 3: 35%-60%
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