Q. No.A piece of DNA, somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of organism if:
| 1. | It includes the centromere |
| 2. | It becomes a part of chromosome |
| 3. | It does not contain any intron sequences |
| 4. | It is a single stranded polynucleotide |
For the multiplication of any alien piece of DNA in an organism, it needs to be part of a chromosome that has a specific:
| 1. | Telomeric sequence | 2. | Multiple cloning sites |
| 3. | Ori | 4. | Selectable marker |
In recombinant DNA experiments, a vector:
| 1. | carries DNA into a new cell |
| 2. | links together newly joined fragments of DN |
| 3. | makes millions of copies of a specific segment of DNA |
| 4. | separates fragments of DNA by their length and electrical charges |
What controls the copy number of linked DNA if plasmid is used as a vector in bacterial transformation?
| 1. | Ori | 2. | rop |
| 3. | Selectable markers | 4. | Promoter in the insert |
What is true for the plasmids used as cloning vectors in recombinant procedures?
| 1. | Ori is responsible for controlling the copy number of linked DNA |
| 2. | The bacteria lacking R plasmids are resistant to antibiotics |
| 3. | The vector must have many sites for the same restriction enzyme |
| 4. | They can carry largest inserts amongst the available cloning vectors |
The most important feature in a plasmid to serve as a vector in gene cloning experiment is:
| 1. | Origin of replication (ori) |
| 2. | Presence of a selectable marker |
| 3. | Presence of sites for restriction endonuclease |
| 4. | Its size |
Some bacteria are resistant to antibiotics. This trait is normally due to:
1. lack of membrane bound organelles
2. presence of a cell wall
3. presence of an extra-chromosomal circular DNA
4. a unique feature called as inclusion bodies
In the given diagram of pBR322, the location of the recognition sequences of BamH I and Sal I are represented by the numbers:
| 1. | 1 and 2 | 2. | 3 and 4 |
| 3. | 6 and 7 | 4. | 5 and 8 |
A cloning vector has two antibiotic resistance genes: tetracycline and ampicillin. A foreign DNA was inserted into the tetracycline gene. Non-recombinant transformants would survive on the medium containing:
| 1. | ampicillin but not tetracycline |
| 2. | tetracycline but not ampicillin |
| 3. | both tetracycline and ampicillin |
| 4. | neither tetracycline nor ampicillin |
1. Agrobacterium tumefaciens
2. Escherichia coli
3. Bacillus thuringiensis
4. Xanthomonas citri
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