The substrate concentration at which the enzyme catalyzed reaction achieves ½ Vmax is its ____ value.

In general, with every 10^oC rise in the temperature [in the range 0^oC to 40^oC], the rate of enzyme catalyzed reaction:
1. Becomes half
2. Gets doubled
3. Remains same
4. There is no such correlation in general

Low temperature:
1. Preserves enzyme in a temporary inactive state
2. Denatures the enzyme
3. Makes enzyme more active
4. Does not have any affect on enzyme activity