Silencing of specific mRNA is possible via RNAi because of -
1. Complementary tRNA
2. Non-complementary ssRNA
3. Complementary dsRNA
4. Inhibitory ssRNA
Subtopic:  Other GM Crops |
 76%
Level 2: 60%+
NEET - 2025
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Following are the steps involved in action of toxin in Bt Cotton:
A. The inactive toxin converted into active form due to alkaline pH of gut of insect
B. Bacillus thuringiensis produce crystals with toxic insecticidal proteins.
C. The alkaline pH solubilises the crystals.
D. The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect.
E. The toxin proteins exist as inactive protoxins in bacteria.
Choose the correct sequence of steps from the options given below:
1. E→C→B→A→D
2. B→C→A→E→D
3. A→E→B→D→C
4. B→E→C→A→D
Subtopic:  Bt Crops |
 75%
Level 2: 60%+
NEET - 2024
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The Bt toxin in genetically engineered Bt cotton kills the pest by: 
1. creating pores in the midgut 
2. damaging the respiratory system 
3. degenerating the nervous system 
4. altering the pH of body fluids
Subtopic:  Bt Crops |
 93%
Level 1: 80%+
NEET - 2024
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Important objective of biotechnology in agriculture is to:
1. produce pest resistant varieties of plant.
2. increase nitrogen content in plant.
3. decrease the seed number.
4. increase the plant weight.
Subtopic:  Intro to Agri Applications |
 96%
Level 1: 80%+
NEET - 2024
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Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).

Assertion (A):  The nematode cannot survive in a transgenic host which expresses specific interfering RNA.
Reason (R):  Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell.

In light of the above statement, choose the most appropriate answer from the option given below :

1. (A) is True but (R) is False.
2. (A) is False but (R) is True.
3. Both (A) and (R) are True and (R) is the correct explanation of (A).
4. Both (A) and (R) are True but (R) is not the correct explanation of (A).
Subtopic:  Interference RNA Tech |
 84%
Level 1: 80%+
NEET - 2022
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ds RNA can be used during which one of the following?

1. Gene sequencing 2. Polymerase Chain Reaction
3. Gene silencing 4. Autoradiography
Subtopic:  Interference RNA Tech |
 82%
Level 1: 80%+
NEET - 2022
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RNA interference is used for which of the following purposes in the field of biotechnology?

1. to develop a plant tolerant to abiotic stresses
2. to develop a pest-resistant plant against infestation by nematode
3. to enhance the mineral usage by the plant
4. to reduce post-harvest losses

Subtopic:  Interference RNA Tech |
 91%
Level 1: 80%+
NEET - 2020
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In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because: 
1. Genetically engineered lymphocytes are not immortal cells.
2. Retroviral vector is introduced into these lymphocytes.
3. Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages 
4. Lymphocytes from patient's blood are grown in culture, outside the body.
Subtopic:  Gene Therapy |
 76%
Level 2: 60%+
NEET - 2022
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Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
 

1. mutated gene does not appear on a photographic film as the probe has no complementarity with it.
2. mutated gene does not appear on a photographic film as the probe has complementarity with it.
3. mutated gene partially appears on a photographic film.
4. mutated gene completely and clearly appears on a photographic film.
Subtopic:  Molecular Diagnosis |
 71%
Level 2: 60%+
NEET - 2021
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When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

1. Molecular diagnosis 2. Safety testing
3. Biopiracy 4. Gene therapy
Subtopic:  Gene Therapy |
 69%
Level 2: 60%+
NEET - 2021
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