| A. | The inactive toxin converted into active form due to alkaline pH of gut of insect |
| B. | Bacillus thuringiensis produce crystals with toxic insecticidal proteins. |
| C. | The alkaline pH solubilises the crystals. |
| D. | The activated toxin binds to the surface of midgut cells, creates pores and causes death of the insect. |
| E. | The toxin proteins exist as inactive protoxins in bacteria. |
Given below are two statements: one is labeled as Assertion (A) and the other is labeled as Reason (R).
| Assertion (A): | The nematode cannot survive in a transgenic host which expresses specific interfering RNA. |
| Reason (R): | Nematode-specific gene introduced in the host produces both sense and antisense complementary RNA which initiate RNA interference in the host cell. |
In light of the above statement, choose the most appropriate answer from the option given below :
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
ds RNA can be used during which one of the following?
| 1. | Gene sequencing | 2. | Polymerase Chain Reaction |
| 3. | Gene silencing | 4. | Autoradiography |
RNA interference is used for which of the following purposes in the field of biotechnology?
| 1. | to develop a plant tolerant to abiotic stresses |
| 2. | to develop a pest-resistant plant against infestation by nematode |
| 3. | to enhance the mineral usage by the plant |
| 4. | to reduce post-harvest losses |
| 1. | Genetically engineered lymphocytes are not immortal cells. |
| 2. | Retroviral vector is introduced into these lymphocytes. |
| 3. | Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages |
| 4. | Lymphocytes from patient's blood are grown in culture, outside the body. |
Nowadays, it is possible to detect the mutated gene causing cancer by allowing the radioactive probe to hybridise its complementary DNA in clone of cells, followed by its detection using autoradiography because:
| 1. | mutated gene does not appear on a photographic film as the probe has no complementarity with it. |
| 2. | mutated gene does not appear on a photographic film as the probe has complementarity with it. |
| 3. | mutated gene partially appears on a photographic film. |
| 4. | mutated gene completely and clearly appears on a photographic film. |
When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:
| 1. | Molecular diagnosis | 2. | Safety testing |
| 3. | Biopiracy | 4. | Gene therapy |