NEETprep Bank NEET Zoology Biotechnology and its Application Questions Solved


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The enzyme used in the polymerase chain reaction is a :

(1) DNA dependent RNA polymerase

(2) RNA dependent DNA polymerase

(3) DNA dependent DNA polymerase

(4) RNA dependent RNA polymerase

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Taq polymerase is used in the polymerase chain reaction because:

(1) It replicates DNA faster than other enzymes

(2) It is the only enzyme that can replicate DNA invitro

(3) It does not require an RNA primer to function

(4) It is thermostable 

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Plants are more easily manipulated by genetic engineering than are animals because:

(1) Plant genes do not contain introns

(2) More vectors are available to transfer rDNA into plant cells

(3) Totipotency is a feature generally associated with plant cells and not with animal cells

(4) Genes can be inserted into animal cells by microinjection.

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Identify the incorrectly matched pair :

(1)

 Humulin

First therapeutic rDNA product approved for human use

(2)

RNAi

Silencing of mRNA with the help of dsRNA

(3)

Rosie

Transgenic sheep producing alpha 1 antitrypsin

(4)

Golden rice

Vitamin A enriched rice variety

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RNAi is a mechanism to silence genes with the help of 

(1) ssRNA

(2) dsRNA

(3) ssDNA

(4) dsDNA

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To protect tobacco plants against Meloidegyne incognita using RNAi, nematode specific genes were introduced in the plant using:

(1) Transposons

(2) Retrotransposons

(3) Virus with RNA genome 

(4) Agrobacterium

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A permanent cure for ADA deficiency could be:

(1) Enzyme replacement therapy

(2) Bone marrow transplant

(3) Introduction of a functional ADA cDNA into lymphocytes

(4) Introduction of gene isolate from marrow cells producing ADA into early embryonic cells

 

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Taq polymerase is used in the polymerase chain reaction because:

(1) It replicates DNA faster than other enzymes

(2) It is the only enzyme that can replicate DNA invitro

(3) It does not require RNA primer to function

(4) It is thermostable   

 

 

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Polymerase Chain Reaction is not used in :

(1) Confirming presence of a pathogen during early infection

(2) Identifying the mutated genes in suspected cancer patients

(3) Isolating the gene of interest from host DNA to be cloned by recombinant procedures

(4) Detection of the presence of HIV in suspected AIDS patient

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