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Q. No.How many statements given below are correct with respect to plasmids?
a.
They float freely in the cytoplasm of certain bacterial cells.
b.
They have the ability to replicate, independent of the genomic DNA.
c.
Herbert Boyer developed a method of removing and reinserting them in the cells.
d.
They are circular ssDNA molecules.
Select the correct option.
1. Three
2. Four
3. Two
4. One
Subtopic: Introduction & History |
Level 3: 35%-60%
Hints
Which of the following can act as selectable markers in a cloning vector?
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
a. ampR
b. tetR
c. ori
d. rop
Select the correct option.
1. (a) and (b)
2. (c) and (d)
3. (a), (c) and (d)
4. (a), (b), (c) and (d)
Subtopic: Cloning Vector |
86%
Level 1: 80%+
Hints
Complete the analogy and select the correct option.
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
BamHI : Sticky ends : : EcoRV : ______
1. Staggered ends
2. Cohesive ends
3. Blunt ends
4. Ends with overhanging stretches
Subtopic: Restriction Enzymes - Main Enzymes |
78%
Level 2: 60%+
Hints
Match column I with column II and choose the correct option:
1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
| Column I | Column II | ||
| a. | Salmonella typhimurium | (i) | Source of EcoRI |
| b. | Bacillus thuringiensis | (ii) | Used in developing transgenic tobacco plant |
| c. | Agrobacterium tumefaciens |
(iii) | Used in creating first recombinant DNA |
| d. | Escherichia coli | (iv) | Gene used in creating biopesticide |
1. a(iv), b(i), c(ii), d(iii)
2. a(iii), b(iv), c(i), d(ii)
3. a(i), b(iv), c(ii), d(iii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic: Restriction Enzymes - Main Enzymes | Transforming Plant & Animal Cell | Restriction Enzymes: Historical Background |
87%
Level 1: 80%+
Hints
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| Assertion(A): | If BamHI cuts a 12 kb linear dsDNA fragment at two restriction sites which are at the same distance from the ends and each other, then only one band is obtained on agarose gel electrophoresis. |
| Reason(R): | Three equal sized fragments will be obtained on restriction digestion by BamHI. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
| 3. | (A) is false but (R) is true. |
| 4. | (A) is true but (R) is false |
Subtopic: Tools: Enzymes: I |
Level 3: 35%-60%
Hints
In agarose gel electrophoresis, DNA molecules are separated on the basis of
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
a. Size
b. Charge
c. Charge to size ratio
d. Length of agarose gel
Select the correct option:
1. a only
2. b only
3. a, b and c
4. a, b, c and d
Subtopic: Separation and Isolation of DNA fragments |
81%
Level 1: 80%+
Hints
Match Column-I with Column-II and select the correct option:
1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
| Column-I | Column-II | ||
| a. | Restriction endonuclease | (i) | Hind II |
| b. | First recombinant DNA | (ii) | Obtained from strain RY13 |
| c. | First restriction endonuclease | (iii) | Isolated in 1963 |
| d. | EcoRI | (iv) | Developed in 1972 |
1. a(iii), b(i), c(ii), d(iv)
2. a(i), b(ii), c(iii), d(iv)
3. a(iii), b(iv), c(i), d(ii)
4. a(iii), b(iv), c(ii), d(i)
Subtopic: Restriction Enzymes: Historical Background | Introduction & History |
82%
Level 1: 80%+
Hints
Read the following statements and choose the correct option.
1. Only statement A is correct
2. Only statement A is incorrect
3. Both A and B statements are correct
4. Both A and B statements are incorrect
| Statement (A): | DNA cannot pass through cell membranes as it is a hydrophobic molecule. |
| Statement (B): | Treatment with Ca2+ ions increases the efficiency with which DNA enters into the bacterium. |
2. Only statement A is incorrect
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Transforming Plant & Animal Cell |
64%
Level 2: 60%+
Hints
Match column-I with column-II and choose the correct option:
1. a(iv), b(ii), c(iii), d(i)
2. a(iv), b(ii), c(i), d(iii)
3. a(ii), b(iv), c(i), d(iii)
4. a(ii), b(iv), c(iii), d(i)
| Column-I | Column -II | ||
| a. | Biosynthetic stage | (i) | Increased surface area for oxygen transfer |
| b. | Downstream processing | (ii) | Large-scale production in the fermenter |
| c. | Sparged stirred-tank bioreactor |
(iii) | With foam breaker and flat bladed impeller |
| d. | Simple stirred-tank bioreactor |
(iv) | Separation and purification |
1. a(iv), b(ii), c(iii), d(i)
2. a(iv), b(ii), c(i), d(iii)
3. a(ii), b(iv), c(i), d(iii)
4. a(ii), b(iv), c(iii), d(i)
Subtopic: Bioreactors/Downstream Processing | Large Scale Production |
73%
Level 2: 60%+
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Read the following statements and choose the correct option
Statement A: CaCl2 treatment increases the efficiency of DNA uptake to produce transformed bacterial cells
Statement B: The divalent Ca+2 ions create transient pores in the bacteria
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Statement A: CaCl2 treatment increases the efficiency of DNA uptake to produce transformed bacterial cells
Statement B: The divalent Ca+2 ions create transient pores in the bacteria
1. Only statement A is correct
2. Only statement B is correct
3. Both A and B statements are correct
4. Both A and B statements are incorrect
Subtopic: Transforming Plant & Animal Cell |
Level 4: Below 35%
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