Q. No.A. Restriction enzymes
B. DNA ligase
C. DNA mutase
D. DNA recombinase
E. DNA polymerase
Choose the correct answer from the options given below:
| 1. | D and E only | 2. | B and C only |
| 3. | C and D only | 4. | A and B only |
Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
| 1. | Ori site | 2. | Palindromic sequence |
| 3. | Recognition site | 4. | Selectable marker |
The specific palindromic sequence which is recognized by EcoRI is:
| 1. | 5' -GGAACC-3' 3'-CCTTGG-5' |
2. | 5'-CTTAAG-3' 3'-GAATTC-5' |
| 3. | 5'-GGATCC-3' 3'-CCTAGG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
Match the following enzymes with their functions:
|
Column-I |
Column-II |
||
|
(a) |
Restriction endonuclease |
(i) |
joins the DNA fragments |
|
(b) |
Exonuclease |
(ii) |
extends primers on genomic DNA template |
|
(c) |
DNA ligase |
(iii) |
cuts DNA at a specific position |
|
(d) |
Tag polymerase |
(iv) |
removes nucleotides from the ends of DNA |
Select the correct option from the following:
| Options: | (a) | (b) | (c) | (d) |
| 1. | (iii) | (i) | (iv) | (ii) |
| 2. | (iii) | (iv) | (i) | (ii) |
| 3. | (iv) | (iii) | (i) | (ii) |
| 4. | (ii) | (iv) | (i) | (iii) |
An enzyme catalysing the removal of nucleotides from ends of DNA is:
| 1. | DNA ligase | 2. | Endonuclease |
| 3. | Exonuclease | 4. | Protease |
A gene whose expression helps to identify transformed cell is known as:
| 1. | Vector | 2. | Plasmid |
| 3. | Structural gene | 4. | Selectable marker |
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using?
| 1. | Eco Rl | 2. | taq polymerase |
| 3. | polymerase lll | 4. | ligase |
The DNA molecule to which the gene of interest is integrated for cloning is called:
| 1. | Transformer | 2. | Vector |
| 3. | Template | 4. | Carrier |
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