Q. No.
In the above-represented plasmid, an alien piece of DNA is inserted at EcoRI site. Which of the following strategies will be chosen to select the recombinant colonies?
| 1. | White color colonies will be selected. |
| 2. | Blue color colonies grown on ampicillin plates can be selected. |
| 3. | Using an ampicillin- & tetracycline-containing medium plate. |
| 4. | Blue color colonies will be selected. |
Subtopic: Cloning Vector |
61%
Level 2: 60%+
NEET - 2025
Please attempt this question first.
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Please attempt this question first.
The following diagram showing restriction sites in E.coli cloning vector pBR322. Find the role of 'X' and 'Y' genes :
| 1. | The gene 'X' is responsible for controlling the copy number of the linked DNA and 'Y' for protein involved in the replication of Plasmid. |
| 2. | The gene 'X' is 'for protein involved in replication of Plasmid and 'Y' for resistance to antibiotics. |
| 3. | Gene 'X' is responsible for recognition sites and 'Y' is responsible for antibiotic resistance. |
| 4. | The gene 'X' is responsible for resistance to antibiotics and 'Y' for protein involved in the replication of Plasmid. |
Subtopic: Cloning Vector |
76%
Level 2: 60%+
NEET - 2024
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Which of the following is not a selectable marker of cloning vectors?
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
1. Ampicillin
2. Metformin
3. Chloramphenicol
4. Tetracycline
Subtopic: Cloning Vector |
89%
Level 1: 80%+
NEET - 2024
Hints
Select the restriction endonuclease enzymes whose restriction sites are present for the tetracycline resistance (tetR) gene in the pBR322 cloning vector.
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
1. Bam HI and Sal I
2. Sal I and Pst I
3. Pst I and Pvu I
4. Pvu I and Bam HI
Subtopic: Cloning Vector |
86%
Level 1: 80%+
NEET - 2024
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Ligation of foreign DNA at which of the following site will result in loss of tetracycline resistance of pBR322?
| 1. | PsT I | 2. | Pvu I |
| 3. | EcoR I | 4. | BamH I |
Subtopic: Cloning Vector |
86%
Level 1: 80%+
NEET - 2023
Hints
Match List-I with List-II
Choose the correct answer from the options given below:
| List-I | List-II | ||
| (A) | Kanamycin | (I) | Delivers genes into animal cells |
| (B) | ClaI | (II) | Selectable marker |
| (C) | Disarmed retroviruses | (III) | Restriction site |
| (D) | Kanamycin Rgene | (IV) | Antibiotic resistance |
Choose the correct answer from the options given below:
| Options: | (A) | (B) | (C) | (D) |
| 1. | (II) | (III) | (I) | (IV) |
| 2. | (III) | (I) | (IV) | (II) |
| 3. | (IV) | (III) | (I) | (II) |
| 4. | (II) | (IV) | (I) | (III) |
Subtopic: Cloning Vector |
55%
Level 3: 35%-60%
NEET - 2023
Hints
Which of the following is not a desirable feature of a cloning vector?
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
1. Presence of two or more recognition sites
2. Presence of origin of replication
3. Presence of a marker gene
4. Presence of single restriction enzyme site
Subtopic: Cloning Vector |
75%
Level 2: 60%+
NEET - 2022
Hints
Identify the properties of a good vector used in rDNA technology:
| (a) | It should have origin of replication supporting a high copy number |
| (b) | It should have preferably more than '2' recognition sites |
| (c) | The restriction sites in vector should be in the antibiotic- resistant genes |
| (d) | It should have suitable marker genes |
| (e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
| 1. | (a), (c) and (e) only | 2. | (c), (d) and (e) only |
| 3. | (a), (b) and (c) only | 4. | (a), (c), (d) and (e) only |
Subtopic: Cloning Vector | Tools |
87%
Level 1: 80%+
NEET - 2022
Hints
Pathogenic bacteria gain resistance to antibiotics due to changes in their:
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
1. Cosmids
2. Plasmids
3. Nucleus
4. Nucleoid
Subtopic: Cloning Vector |
86%
Level 1: 80%+
NEET - 2022
Hints
Plasmid pBR322 has a PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for -galactoside production and the recombinant plasmid is inserted in an E.coli strain,
| 1. | it will lead to the lysis of host cells. |
| 2. | it will be able to produce a novel protein with dual abilities. |
| 3. | it will not be able to confer ampicillin resistance to the host cell. |
| 4. | the transformed cells will have the ability to resist ampicillin as well as produce β -galactoside. |
Subtopic: Cloning Vector |
59%
Level 3: 35%-60%
NEET - 2021
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