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Q. No.Which of the following is not required in the preparation of a recombinant DNA molecule?
1. Restriction endonuclease
2. DNA ligase
3. DNA fragments
4. E. coli
Subtopic: Â General Design of an rDNA experiment |
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The role of DNA ligase in the construction of a recombinant DNA molecule is
| 1. | formation of phosphodiester bond between two DNA fragments |
| 2. | formation of hydrogen bonds between sticky ends of DNA fragments |
| 3. | ligation of all purine and pyrimidine bases |
| 4. | None of the above |
Subtopic: Â General Design of an rDNA experiment |
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A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
| 1. | human genes may have intron which bacteria cannot process |
| 2. | amino acid codons for humans and bacteria are different |
| 3. | human protein is formed but degraded by bacteria |
| 4. | All of the above |
Subtopic: Â General Design of an rDNA experiment |
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