Which of the following has popularised the PCR (Polymerase Chain Reaction)?
1. Easy availability of DNA template
2. Availability of synthetic primers.
3. Availability of cheap deoxyribonucleotides
4. Availability of ‘Thermostable’ DNA polymerase

An antibiotic resistance gene in a vector usually helps in the selection of

1. Competent bacterial cells                             
2. Transformed bacterial cells
3. Recombinant bacterial cells                           
4. None of the above

Significance of 'heat shock' method in bacterial transformation is to facilitate:
 

The role of DNA ligase in the construction of a recombinant DNA molecule is

Which of the following is not a source of restriction endonuclease?
1. Haemophilus influenzae                         
2.Escherichia coli
3. Entamoeba coli                         
4. Bacillus amyloliquefaciens

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
1. Denaturation of template DNA
2. Annealing of primers to template DNA
3. Extension of primer end on the template DNA
4. All of the above

A bacterial cell was transformed with a recombinant DNA that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be:
 

Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?

1. Laboratory flask of largest capacity
2. A stirred-tank bioreactor without in-lets and outlets
3. A continuous culture system
4. Any of the above

Who among the following was awarded the Nobel Prize for the development of the PCR technique?

1. Herbert Boyer                                   
2. Hargovind Khurana
3. Kary Mullis                                       
4. Arthur Kornberg

Which of the following statements does not hold true for restriction enzymes?