Which of the following options is correct about Agrobacterium tumifaciens?

1.     It is a pathogen of angiosperms

2.     It is able to deliver a piece of DNA known as T-DNA to transform bacterial cell and direct the cell to produce chemicals required by the pathogen

3.     Its Ti plasmid is now modified into an expression vector

4.     Its Ti plasmid is modified into a cloning vector which is no more pathogenic

In order to make the host cell competent

Divalent cations such as calcium are used-

1.      The divalent ions have to be in a specific concentration

2.      They cause the DNA uptake by the cell

3.      They increase the efficiency with which DNA enters the bacterium through pores in its cell wall.

4.     All of the above

The process of competent cell formation include

1.      CaCl2 treatment- ice incubation with r-DNA, heat shock at 42 degree Celsius, ice incubation

2.      Ice incubation - CaCl2 treatment - Heat shock-Ice incubation with r-DNA

3.      CaCl2 treatment - Heat shock -Ice incubation with r-DNA

4.     Ice incubation - CaCl2 treatment - Heat treatment at 45 degree Celsius, Ice incubation with r-DNA

For the synthesis of 4 copies of DNA, how many sets of primers are needed in PCR

1.      2 sets

2.      8 sets

3.      4 sets

4.     Random

Arrange the following steps in order:

1) Restriction Digestion
2) Running Agarose gel
3) Elution of bands
4) Purification of DNA
5) ligation
6) transformation
7) Cloning

1.      1-2-3-4-5-6-7

2.      1-5-6-7-2-3-4

3.      1-2-3-5-6-7-4

4.      2-3-4-1-5-6-7

Which of the following step is last for isolating the desired DNA fragment?

1.      Running of gel

2.      Elution

3.      Staining

4.     Visualization

Which of the following step is first one in order to separate DNA fragments?

1.      Running of gel

2.      Staining of gel

3.      Elution

4.     Setting of gel

What does cloning mean?

1.      The ability to multiply the desired gene in the host

2.      The ability to multiply the vector in the host

3.      The ability to multiply the antibiotic resistance gene in the host

4.     The ability to multiple Ori in the host

Cohen and Boyer are known for which of the following discoveries?

1.      Antibiotic resistance gene

2.      Recombinant DNA

3.      Restriction Digestion

4.     Plasmid

If you transform a cell with an alien piece of DNA only, What is the possibility?

1.      This alien piece becomes a part of the host genome.

2.      It doesn't become a part of the host's genome but keeps on replicating

3.      The cloning of genes won't get affected in the absence of Origin of replication.

4.     Multiple identical copies of an alien piece of DNA can be formed irrespective of its integration into the host's genome.