The vector used to transfer gene to produce pest resistant tobacco plant is

(1) pBR 322        

(2) pUC 18

(3) Ti plasmid    

(4) λ phage

Which of the following is/are not indirect method/ s of gene transfer?

a. Bacteriophages

b. Plasmids

c. Mobile genetic elements (transposons)

d. Chemical mediated gene transfer

e. Electroporation

Options

(1) a & b              

(2) b & c

(3) d & e              

(4) c, d & e

Following enzymes/techniques are used in the process of recombinant DNA technology

a. EcoRl to cut the isolated genome

b. DNA ligase

c. Protease and ribonuclease for removal of proteins and RNA from DNA

d. Production of recombinant hosts

e. Lysozyme for isolation of the genetic material (DNA)

f. Gel electrophoresis for separation and isolation of DNA fragments

Mark the correct sequence of their use

(1) c, e, b, f, a, d               

(2) e, c, a, b, f, d

(3) e, c, a, f, b, d

(4) a, e, c, b, d, f

Following are the steps in the formation of recombinant DNA by action of a restriction endonuclease. Which one of the given option incorrectly identifies the steps, components labelled as A, B, C, and D?

(1) A - Exonuclease cuts the DNA between bases G and A only when the sequence GAATTC is present in the DNA

(2) B - Sticky ends are formed.

(3) C - The DNA fragments join at sticky ends by DNA ligase.

(4) D - Restriction enzyme Eco RI cuts the strand of DNA a little away from centre of the palindromic sites, but between the same two bases on the opposite strands

Which of the following statements is incorrect with respect to polymerase chain reaction?

(1) Repeated amplification is achieved by the use of thermostable DNA polymerase

(2) Thermostable DNA polymerase is isolated from a bacterium, Thermus aquaticus

(3) Each cycle in PCR has three steps, first is primer annealing, second is denaturation and third is extension of primers

(4) In polymerase chain reaction, multiple copies of gene of interest are synthesized in vitro using two sets of primers and enzyme DNA polymerase

The figure below shows three steps (A, B, C) of polymersase chain reaction (PCR). Select the correct identification together with what it represents

1. B - Denaturation at a temperature of about 98°C separating the two DNA strands

2. A - Denaturation at a temperature of about 60°C

3. C - Extension in the presence of heat stable DNA polymerase

4. A - Annealing with two sets of primer

Method of gene transfer that involves use of electrical impulses of high field strength to increase permeability of membrane by creating transient pores in it, is

(1) Microinjection           

(2) Electroporation

(3) Biolistics       

(4) Lipofection

Given below are the three basic steps in genetically modifying an organism

a. Introduction of the identified DNA into the host.

b. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.

c. Identification of DNA with desirable genes.

Choose the option with respect to correct sequence of the given steps

(1) a → c → b    

(2) c → b → a

(3) c → a → b    

(4) b → a → c

'Insertional inactivation' is used for

(1) Selection of recombinants

(2) Producing pest resistant crops

(3) Enhancing nutritional value of food

(4) Reducing post harvest losses

The construction of the first recombinant DNA by linking antibiotic resistance gene with a native plasmid of Salmonella typhimurium was done by

(1) Arber and Nathans

(2) Kary Mullis

(3) Herbert Boyer and Stanley Cohen

(4) Smith and Wilcox