In the above diagram showing a typical agaro gel
electrophoresis

1. Lane 1 shows undigested DNA fragment

2. Lane 4 shows largest DNA particles towards ‘y’

3. The procedure helps to form clones of DNA

4. The procedure takes place as the DNA fragments
are double stranded

Bacterial colonies that have a foreign DNA fragment inserted into the plasmid will appear white because-

1.  X-gal can be cleaved by $\beta$-galactosidase

2.  Lac-Z gene shows insertional inactivation due to insertion of foreign DNA

3.  Lac-Z gene is active

4.  X-gal can be cleaved by permease

Restriction enzymes are synthesized by:

1. Bacteria only

2. Yeast and bacteria only

3. Eukaryotic cells only

4. All kinds of cells

Today we know more than …….. restriction enzymes that have been isolated from over……..strains

1. 700; 350

2. 900; 230

3. 600; 270

4. 550; 210

In a DNA gel the fragment of 2kb and 3kb will be

1. 2kb nearer to cathode while 3kb nearer to anode

2. 2kb closer to wells while 3kb towards the opposite ends

3. 2kb closer to opposite end of gel while 3kb closer to well

4. 2kb away from cathode while 3kb towards anode

rop segment codes for pBR322?

1. Resistance protein

2. Protein for replication

3. Proteins for translation

4. Cloning site

The synthesis of DNA in PCR will be in

1. 5’-3’

2. 3’-5’

3. It won't be depending on direction

4. For one strand 5’-3’ in another opposite to it

The primers designed according to (Answer according to very basic PCR)

1. Terminal ends of vector

2. Terminal ends of gene of interest

3. Random primers

4. According to direction of PCR

Continuous culture leads to

1. Smaller biomass

2. Higher yields of desired proteins

3. Both A and B

4. Higher yields of recombinant DNA

The Agitation system of bioreactor has

1. Flat bladed impeller

2. Sharp impellers

3. Foam breaker

4. Steamer