DNA fragments separated by gel electrophoresis are shown. Mark the correct statement :

 

1.	Band 3 contains more positively charged DNA molecules than band 1.
2.	Band 3 indicates more charge density than bands 1 and 2.
3.	Band 1 has longer DNA fragment than bands 2 and 3.
4.	All bands have equal lengths and charges but differ in base composition.

Which of the following is not a direct method of gene transfer?

1. Microinjection
2. Biolistic
3. Electroporation
4. Disarmed pathogen vector

The restriction endonuclease breaks bonds between:

1. DNA-RNA hybrid

2. Introns

3. Nucleotides, i.e., breaks the phosphodiester bond

4. Pentose sugar and nitrogenous base, i.e., breaks N-glycosidic bond

During gel electrophoresis for separation of DNA fragment:

1. Smallest fragment will move to the farthest point towards cathode

2. Smallest fragment will move to the farthest point towards anode

3. Largest fragment will move to the farthest point towards cathode

4. Largest fragment will move to the farthesr point towards anode

When we ligate a foreign DNA at the sal I site of tetracycline resistance gene in a vector pBR 322, the ____a____ plasmid will lose tetracycline resistance due to insertion of foreign DNA but can still be selected out from ____b____ ones by plating the ___c____ on ampicillin containing medium

        a                                b                                   c

1. Non-recombinant     Recombinant                 Transformant

2. Recombinant           Non-recombinant            Transformant

3. Transformant           Non-recombinant            Recombinant

4. Recombinant            Transformant                  Non-recombinant

If Pvu I restriction endonuclease is used for cleaving pBR322 cloning vector for inserting foreign DNA, which of the following can be expected in recombinants?

1. They remain resistant to tetracycline but not to ampicillin

2. They would die in tetracycline containing medium but remain alive in ampicillin containing medium

3. Replication of recombinant plasmid get prevented

4. They do not show resistance to ampicillin as well as tetracycline

If the gene of interest is cloned at ECoRI in pBR322,
the recombinant E-coli after transformation are

1. Susceptible to ampicillin and tetracyline.
2. Sensitive to tetracycline.
3. Resistant to kanamycin.
4. Resistant to ampicillin and tetracycline

Which of the following enzyme is involved in the
isolation of genetic material like DNA from a plant cell?
I. Cellulase                        II. Lysozyme
III. Ribonuclease               IV. Protease

1. I, II, III and IV 
2. I and II only
3. I only 
4. I, III and IV

Similarity between microinjection and biolistic gene
gun method is
1. Used to transform the plant host cells.
2. Used to transform the animal host cells.
3. Recombinant DNA is directly injected into
host cells.
4. Used to transform the bacterial host cells

The product has to be subjected through a series of processes before it is ready for marketing as a finished product called down stream processing. It includes
1. Separation
2. Purification
3. Quality control
4. Both 1 & 2