Manipulation of DNA in genetic engineering became possible due to the discovery of:

1. Restriction endonuclease

2. DNA ligase

3. Transcriptase

4. Primase

Subtopic:  Restriction Enzymes - Main Enzymes |
 91%
From NCERT
AIPMT - 2002
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Restriction endonucleases:

1.  Are used in genetic engineering for ligating two DNA molecules.
2.  Are used for in vitro DNA synthesis.
3.  Are synthesized by bacteria as part of their defense mechanism.
4.  Are present in mammalian cells for the degradation of DNA when the cell dies. 

Subtopic:  Restriction Enzymes - Main Enzymes |
 55%
From NCERT
AIPMT - 2004
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Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain:

1. Lipase

2. protease

3. Exonuclease

4. Endonuclease

Subtopic:  Restriction Enzymes - Main Enzymes |
 78%
From NCERT
AIPMT - 2005
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