Q. No.Which of the following is most used in genetic engineering:
1. E. coil and Agrobacterium
2. Mycobacteria and Salmonella
3. Aspergillus
4. Penicillium
1. E. coil and Agrobacterium
2. Mycobacteria and Salmonella
3. Aspergillus
4. Penicillium
You are required to draw blood from a patient and to keep it in a test tube for analysis of blood corpuscles and plasma. You are also provided with the following four types of test tubes. Which of them will you not use for the purpose?
1. Chilled test tube
2. Test tube containing heparin
3. Test tube containing sodium oxalate
4. Test tube containing calcium bicarbonate
Restriction endonucleases:
| 1. | Are used in genetic engineering for ligating two DNA molecules. |
| 2. | Are used for in vitro DNA synthesis. |
| 3. | Are synthesized by bacteria as part of their defense mechanism. |
| 4. | Are present in mammalian cells for the degradation of DNA when the cell dies. |
The Ti plasmid is often used for making transgenic plants. This plasmid is found in:
1. Rhizobium of the roots of leguminous plants
2. Agrobacterium
3. Yeast as a 2 µm plasmid
4. Azotobacter
Function of Nucleases:
| 1. | Break the polynucleotide chain by breaking each terminal nucleotide |
| 2. | Breaks phosphodiester bond |
| 3. | Breaks peptide bonds |
| 4. | Breaks ester bonds |
Hybridoma is:
| 1. | Collection of DNA from DNA |
| 2. | Collection of RNA from DNA |
| 3. | A fusion of tumour sex cell with non tumour sex cell |
| 4. | A fusion of tumour somatic cell with non tumour somatic cell |
The bacteria generally used for genetic engineering is:
1. Agrobacterium
2. Bacillus
3. Pseudomonas
4. Clostridium
A giant rat is formed in the laboratory, what is the reason:
1. Gene mutation
2. Gene synthesis
3. Gene manipulation
4. Gene replication
Plasmid has been used as a vector because:
| 1. | It is circular DNA that has the capacity to join to eukaryotic DNA |
| 2. | It can move between prokaryotic and eukaryotic cells |
| 3. | Both ends show replication |
| 4. | It has an antibiotic resistance gene |
In bacteria, the plasmid is:
1. Extrachromosomal material
2. Main DNA
3. Non-functional DNA
4. Repetitive gene
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