Using imprints from a plate with a complete medium and carrying bacterial colonies, you can select streptomycin-resistant mutants and prove that such mutations do not originate as adaptation. These imprints need to be used:

1. On plates with and without streptomycin

2. Only on plates with streptomycin

3. On plates with minimal medium

4. Only on plates without streptomycin 

Which one of the following hydrolyses internal phosphodiester bonds in a polynucleotide chain:

1. Lipase

2. protease

3. Exonuclease

4. Endonuclease

DNA of E. Coli:
1.  ds circular
2.  ss circular
3.  ds Linear
4.  ss Linear

Knife of DNA:
1. DNA–ligase
2. Restriction endonuclease
3. Exonuclease
4. Peptidase

Genetic engineering involves:
1. Use of restriction endonuclease on bacterial DNA and formation of new traits
2. Use of Ligase for cutting DNA
3. Developing instruments
4. Use of statistic in genetics

Which of the following is most used in genetic engineering:
1.  E. coil and Agrobacterium
2.  Mycobacteria and Salmonella
3.  Aspergillus
4.  Penicillium

You are required to draw blood from a patient and to keep it in a test tube for analysis of blood corpuscles and plasma. You are also provided with the following four types of test tubes. Which of them will you not use for the purpose?

1. Chilled test tube

2. Test tube containing heparin

3. Test tube containing sodium oxalate

4. Test tube containing calcium bicarbonate

Restriction endonucleases:

The Ti plasmid is often used for making transgenic plants. This plasmid is found in:

1. Rhizobium of the roots of leguminous plants

2. Agrobacterium

3. Yeast as a 2 µm plasmid

4. Azotobacter

Function of Nucleases: