The DNA molecule to which the gene of interest is integrated for cloning is called:

1. Transformer 2. Vector
3. Template 4. Carrier 
Subtopic:  Tools: Vectors: I | Tools |
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The cutting of DNA at specific locations became possible with the discovery of

1. Restriction enzymes

2. probes

3. Selectable markets

4. ligases

Subtopic:  Tools: Enzymes: II | Tools |
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The introduction of t-DNA into plants involves

1. Infection of the plant by Argobacterium tumefaciens

2. Altering the pH of soil, heat-shocking the plants

3. Exposing the plants to Cold for a brief period

4. Allowing the plant roots to stand in water

Subtopic:  Tools: Vectors: II | Tools |
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Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Subtopic:  Tools: Vectors: I | Tools |
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The figure below is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)? 

1. Ori-original restriction enzyme

2. Rop-reduced osmotic pressure

3. Hind III, Eco RI-selectable markers

4. AmpR, tetiR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
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Which one of the following is a case of wrong matching?

1. Somatic hybridization- Fusion of two diverse cells

2. Vector DNA- Site for tRNA synthesis

3. Micropropagation- In vitro production of plants in large numbers

4. Callus - Unorganized mass of cells produced in tissue culture

Subtopic:  Tools: Vectors: I | Tools |
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Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it? 
5’___GAATTC___3’ 
3’___CTTAAG__5’

1.  Deletion mutation

2.  Start codon at the 5' end

3.  Palindromic sequence of base pairs

4.  Replication completed

Subtopic:  Tools: Enzymes: II | Restriction Enzymes - Main Enzymes | Tools |
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The linking of the antibiotic resistance gene with the plasmid vector became possible with

1.  DNA ligase

2.  endonucleases

3.  DNA polymerase

4.  exonucleases

Subtopic:  Tools: Enzymes: II | Restriction Enzymes - Main Enzymes | Separation and Isolation of DNA fragments | Tools |
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