The figure below is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)? 

1. Ori-original restriction enzyme

2. Rop-reduced osmotic pressure

3. Hind III, Eco RI-selectable markers

4. Amp^R, teti^R-antibiotic resistance genes

Which of the following are used in gene cloning-

1. Lomasomes

2. Mesosomes

3. Plasmids

4. Nucleoids

In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:

A. a bacterium

B. plasmid

C. plasmodium

D. bacteriophage

Options :
1. (A) only
2. (A) and (C) only
3. (B) and (D) only
4. (A), (B) and (D) only