Which vector can clone only a small fragment of DNA?

1. Bacterial artificial chromosome

2. Yeast artificial chromosome

3. Plasmid

4. Cosmid

Subtopic:  Tools: Vectors: I | Tools |
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Which of the following is not correctly matched for the organism and its cell wall degrading enzyme?

1. Plant cells- cellulose

2. Algae - Methylase

3. Fungi - Chitinase

4. Bacteria - Lysozyme

Subtopic:  Obtaining Copy of Gene from Donor DNA |
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DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by :

1. Polymerase chain reaction

2. Electrophoresis

3. Restriction mapping

4. Centrifugation

Subtopic:  Separation and Isolation of DNA fragments |
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The colonies of recombinant bacteria appear white because of:

1. Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
2. Insertional inactivation of beta-galactosidase in recombinant bacteria
3. Inactivation of glycosidase enzyme in recombinant bacteria
4. Non-recombinant bacteria containing beta-galactosidase
Subtopic:  Selectable Markers: III | Selection of Recombinant Transformants | Selectable Markers |
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The figure below is the diagrammatic representation of the E. coli vector pBR 322. Which one of the given options correctly identifies its certain components (s)? 

1. Ori-original restriction enzyme

2. Rop-reduced osmotic pressure

3. Hind III, Eco RI-selectable markers

4. AmpR, tetiR-antibiotic resistance genes

Subtopic:  Tools: Vectors: II | Cloning Vector | Tools |
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Which one of the following is a case of wrong matching?

1. Somatic hybridization- Fusion of two diverse cells

2. Vector DNA- Site for tRNA synthesis

3. Micropropagation- In vitro production of plants in large numbers

4. Callus - Unorganized mass of cells produced in tissue culture

Subtopic:  Tools: Vectors: I | Tools |
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Which one is a true statement regarding DNA polymerase used in PCR?

1. It is used to ligate introduces DNA in recipient cells

2. It serves as a selectable marker

3. It is isolated from a virus

4. It remains active at high temperature

Subtopic:  Polymerase Chain Reaction: PCR |
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For transformation, microparticles coated with DNA to be bombarded with gene gun are made up of

1. Silver or platinum

2. Platinum or zinc

3. Silicon or platinum

4. Gold or tungsten

Subtopic:  Transforming Plant & Animal Cell |
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Given below is a sample of portion of DNA strand giving the base sequence on the opposite strands. What is so, special shown in it? 
5’___GAATTC___3’ 
3’___CTTAAG__5’

1.  Deletion mutation

2.  Start codon at the 5' end

3.  Palindromic sequence of base pairs

4.  Replication completed

Subtopic:  Tools: Enzymes: II | Restriction Enzymes - Main Enzymes | Tools |
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There is a restriction endonuclease called Eco RI. 
What does 'co' part in it stand for?

1.  Coelom

2.  Coenzyme

3.  Coli

4.  Colon

Subtopic:  Restriction Enzymes - Main Enzymes |
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