Q. No.In genetic engineering, a DNA segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A-D) in this regard and select the correct option about which one or more of these can be used as a vector/vectors:
A.
a bacterium
B.
plasmid
C.
plasmodium
D.
bacteriophage
Options:
1.
(A) only
2.
(A) and (C) only
3.
(B) and (D) only
4.
(A), (B) and (D) only
Which one of the following techniques made it possible to genetically engineer living organisms?
1. Hybridization
2. Recombinant DNA techniques
3. X-ray diffraction
4. Heavier isotope labelling
Which of the following are used in gene cloning:
1. Lomasomes
2. Mesosomes
3. Plasmids
4. Nucleoids
Biolistics (gene-gun) is suitable for:
| 1. | Transformation of plant cells |
| 2. | Constructing recombinant DNA by joining with vectors |
| 3. | DNA fingerprinting |
| 4. | Disarming pathogen vectors |
Which one of the following represents a palindromic sequence in DNA?
| 1. | 5'-CCAATG-3' 3'-GAATCC-5' |
2. | 5'-CATTAG-3' 3'-GATAAC-5' |
| 3. | 5'-GATACC-3' 3'-CCTAAG-5' |
4. | 5'-GAATTC-3' 3'-CTTAAG-5' |
The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents.
| 1. | A - Denaturation at a temperature of about 50ºC |
| 2. | C - Extension in the presence of heat-stable DNA polymerase |
| 3. | A - Annealing with two sets of primers |
| 4. | B - Denaturation at a temperature of about 98ºC separating the two DNA strands |
In genetic engineering, the antibiotic-resistance gene is used:
1. To select healthy vectors
2. As sequences from where replication starts
3. To keep the cultures free of infection
4. As selectable markers
1. Precipitate DNA
2. Break open the cell to release DNA
3. Facilitate action of restriction enzymes
4. Remove proteins such as histones
Restriction endonuclease:
1. cuts the DNA molecule randomly
2. cuts the DNA molecule at specific sites
3. restricts the synthesis of DNA inside the nucleus
4. synthesis DNA
1. DNA ligase
2. endonucleases
3. DNA polymerase
4. exonucleases
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