What is the criterion for the movement of DNA fragments on agarose gel during gel electrophoresis?

1. The smaller the fragment size, the farther it moves

2. Positively charged fragment move to farther end

3. Negatively charged fragment do not  move 

4. The larger the fragment size, the farther it moves

Subtopic:  Separation and Isolation of DNA fragments |
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Which of the following is a restriction endonuclease?
1. Protease
2. DNase I
3. RNase
4. Hind II
Subtopic:  Tools: Enzymes: II | Tools |
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Which of the following is not a feature of the plasmids ?
1. Circular structure
2. Transferable
3. Single-stranded
4. Independent replication
Subtopic:  Tools: Vectors: I | Tools |
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The Taq polymerase enzyme is obtained from
1. Thiobacillus ferroxidans
2. Bacillius subtilis
3. Pseudomonas putida
4. Thermus aquaticus
Subtopic:  Polymerase Chain Reaction: PCR |
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A gene whose expression helps to identify transformed cell is known as:

1. Vector 2. Plasmid
3. Structural gene 4. Selectable marker
Subtopic:  Selectable Markers: I | Selectable Markers: II | Selectable Markers: III | Selectable Markers |
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DNA fragments are:

1. Negatively charged

2. Neutral

3. Either positively charged depending on their size

4. Positively charged

Subtopic:  Separation and Isolation of DNA fragments |
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Which of the following restriction enzymes produces blunt ends?

1.  Sal l

2.  Eco RV

3.  Xho

4.  Hind lll

Subtopic:  Restriction Enzymes - Main Enzymes |
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

1.  Eco Rl

2.  taq polymerase

3.  polymerase lll

4.  ligase

Subtopic:  Tools: Enzymes: I | Tools |
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Stirred-tank bioreactors have been designed for

1.  purification of product

2.  addition of preservatives to the product

3.  availability of oxygen throughout the process

4.  ensuring anaerobic conditions in the culture vessel

Subtopic:  Large Scale Production: I | Large Scale Production |
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The process of separation and purification of expressed protein before marketing is called:

1. Downstream processing

2. Bioprocessing

3. Post-product processing

4. Upstream processing

Subtopic:  Bioreactors/Downstream Processing |
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