A gene, whose expression helps to identify transformed cells is known as

1. selectable marker                 

2. vector

3. plasmid                                 

4. structural gene

Subtopic:  Selectable Markers: II | Selectable Markers |
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The DNA fragments separated on an agarose gel can be visualised after staining with

(1) bromophenol blue

(2) acetocarmine

(3) aniline blue 

(4) ethidium bromide

Subtopic:  Process of Biotech |
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Stirred-tank bioreactors have been designed for:

(1) purification of product

(2) addition of preservatives to the product

(3) availability of oxygen throughout the process

(4) ensuring anaerobic conditions in the culture vessel

Subtopic:  Large Scale Production: II | Large Scale Production |
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A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using

(1) Eco RI                       

(2) taq polymerase

(3) polymerase III           

(4) ligase

Subtopic:  Tools: Enzymes: II | Tools |
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Which of the following is not a component of downstream processing?

1. Separation

2. Purification

3. Preservation

4. Expression

Subtopic:  Large Scale Production: II | Large Scale Production |
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Which of the following restriction enzymes produces blunt ends?

(1) Sal I                           

(2) Eco RV

(3) Xho                           

(4) Hind III

 

Subtopic:  Tools: Enzymes: II | Tools |
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Which of the following is a endonuclease?

(1) Protease

(2) DNase I

(3) RNase restriction

(4) Hind II

Subtopic:  Tools: Enzymes: II | Tools |
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The Taq polymerase enzyme is obtained from

1. Thiobacillus ferroxidans

2. Bacillus subtilis

3. Pseudomonas subtilis

4. Thermus aquaticus

Subtopic:  Polymerase Chain Reaction: PCR |
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Stirred-tank bioreactors have been designed for

1. addition of preservatives of the product

2. purification of the product

3. ensuring anaerobic conditions in the culture vessel

4. availability of oxygen throughout the process

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What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?

(1) The larger the fragment size, the farther it moves

(2) The smaller the fragment size, the farther it moves

(3) Positively charged fragments move to farther end

(4) Negatively charged fragments do not move

Subtopic:  Process of Biotech |
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