Q. No.The DNA fragments separated on an agarose gel can be visualised after staining with
(1) bromophenol blue
(2) acetocarmine
(3) aniline blue
(4) ethidium bromide
Agarose extracted from sea weeds finds use in
1. tissue culture
2. PCR
3. gel electrophoresis
4. spectrophotometry
What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis ?
| 1. | The larger the fragment size, the farther it moves |
| 2. | The smaller the fragment size, the farther it moves |
| 3. | Positively charged fragments move to farther end |
| 4. | Negatively charged fragments do not move |
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
| 1. | centrifugation |
| 2. | polymerase chain reaction |
| 3. | electrophoresis |
| 4. | restriction mapping |
If any protein-encoding gene is expressed in a heterologous host, it is termed as
1. Recombinant protein
2. Single-cell protein
3. Humulin
4. Transposon
Consider the following statements:
| I: | Asexual reproduction preserves genetic information while sexual reproduction permits variations. |
| II: | Traditional hybridization often leads to the inclusion and multiplication of undesirable genes along with the desired genes. |
| III: | rDNA technology allows us to isolate and introduce only one or a set of desirable genes without introducing undesirable genes in the target organism. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
The enzyme that seals 5’ PO4 and 3’ OH polynucleotide ends while creating a recombinant DNA molecule is
1. Alkaline phosphatase
2. DNA ligase
3. DNAse
4. Restriction endonuclease
In a DNA gel the fragment of 2kb and 3kb will be
1. 2kb nearer to cathode while 3kb nearer to anode
2. 2kb closer to wells while 3kb towards the opposite ends
3. 2kb closer to opposite end of gel while 3kb closer to well
4. 2kb away from cathode while 3kb towards anode
The visualization of the DNA bands on the gel can be done with the help of
(1) UV rays
(2) Ethidium bromide
(3) Chromogenic substrate
(4) More than one is correct
Given below are four statements pertaining to the separation of DNA fragments using gel electrophoresis. Identify the incorrect statements:
| (a) | DNA is a negatively charged molecule and so it is loaded on gel towards the anode terminal. |
| (b) | DNA fragments travel along the surface of the gel, whose concentration does not affect the movement of DNA. |
| (c) | The smaller the DNA fragment, the greater the distance it travels through it. |
| (d) | Pure DNA can be visualized directly by exposing it to UV radiation. |
Choose the correct answer from the options given below:
| 1. | (a), (c) and (d) | 2. | (a), (b) and (c) |
| 3. | (b), (c) and (d) | 4. | (a), (b) and (d) |
© 2025 GoodEd Technologies Pvt. Ltd.