During translation initiation in prokaryotes, a GTP molecule is needed in:
| 1. | Formation of formyl-met-tRNA |
| 2. | Binding of 30S subunit of the ribosome with mRNA |
| 3. | Association of 30 S-mRNA with formyl met-tRNA |
| 4. | Association of 50 S subunit of the ribosome with the initiation complex |