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Q. No.Traditional hybridisation procedure used in plant and animal breeding:
| 1. | is not useful in production of improved variants. |
| 2. | preserves the genetic information in populations. |
| 3. | is a very fast process to produce large number of progeny in a very short time. |
| 4. | very often leads to inclusion and multiplication of undesirable genes along with the desired genes. |
Subtopic: Introduction & History |
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The breakthrough work of which of the following scientist duo was the basis upon which the discipline of biotechnology was founded?
1. Nirenberg and Mathai
2. Cohen and Boyer
3. Millstein and Kohler
4. Khurana and Venkatraman
1. Nirenberg and Mathai
2. Cohen and Boyer
3. Millstein and Kohler
4. Khurana and Venkatraman
Subtopic: Principles of Biotechnology |
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Which of the following would NOT be a useful selectable marker?
| 1. | A gene encoding a protein that degrades the antibiotic ampicillin. |
| 2. | A gene encoding a protein that allows the cell to synthesize histidine. |
| 3. | A gene encoding a protein that is an essential structural component of the cell. |
| 4. | All of these are useful selectable markers. |
Subtopic: Selectable Markers: I | Selectable Markers: II | Selectable Markers |
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Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
| 1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
| 2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
| 3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
| 4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
Subtopic: Host & Desired DNA: II | Process of Biotech | Host & Desired DNA |
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Consider the following statements:
| I: | Ori is responsible for the copy number of the linked DNA. |
| II: | Transformation is a process through which a piece of DNA is introduced into a host bacterium. |
| III: | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
| 1. | I and II only | 2. | I and III only |
| 3. | II and III only | 4. | I, II, and III |
Subtopic: Tools: Vectors: I | Selectable Markers: I | Selectable Markers | Tools |
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| Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
| Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
| 1. | Both (A) and (R) are True and the (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True but the (R) is not the correct explanation of (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
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| Assertion (A): | When constructing rDNA, the vector DNA should never be cut by the same restriction endonuclease that was used to cut the donor DNA |
| Reason (R): | Use of same restriction endonuclease will generate same kind of sticky ends retarding the function of DNA ligase. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A) |
| 3. | (A) is True but (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
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Consider the given two statements:
| Assertion(A): | A piece of DNA, which is somehow transferred into an alien organism, will be able to multiply itself in the progeny cells of the organism. |
| Reason (R): | All DNA molecules are capable of autonomously replicating. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Cloning Vector |
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Consider the given two statements:
| Assertion(A): | Plasmid is a suitable cloning vector that can be used in host bacterial cells. |
| Reason (R): | Plasmids are extra-chromosomal circular double stranded DNA molecules |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Tools: Vectors: I | Restriction Enzymes - Main Enzymes |
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Consider the given two statements:
| Assertion (A): | Restriction enzymes are found in bacteria and provide a defense mechanism against invading viruses. |
| Reason (R): | They interact with membranes of the host bacterial cells and remove all the receptor sites by the viruses. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A). |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 3. | (A) is True; (R) is False |
| 4. | Both (A) and (R) are False |
Subtopic: Restriction Enzymes - Main Enzymes |
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