Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?

1.  restriction endonuclease digestion of cellular and plasmid DNAs.
2.  production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs.
3.  separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates.
4.  transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin.
Subtopic:  Host & Desired DNA: II | Process of Biotech | Host & Desired DNA |
 53%
From NCERT
Please attempt this question first.
Hints
Please attempt this question first.

Identify the incorrect statement:
1. The process of separation and purification of expressed protein before marketing is called as downstream processing
2. DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion
3. Stirred-tank bioreactors have been designed for availability of oxygen throughout the process
4. Reporter genes can be used as markers for selecting successfully transformed cells
Subtopic:  Process of Biotech |
 63%
From NCERT
Please attempt this question first.
Hints
Please attempt this question first.