Q. No.Consider the given two statements:
| Assertion (A): | Repetitive satellite DNA sequences are very useful identification tools in forensic applications. |
| Reason (R): | These sequences normally do not code for any proteins, but they form a large portion of human genome. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is True but (R) is False. |
| 3. | (A) is False but (R) is True. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: DNA Fingerprinting |
57%
From NCERT
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Satellite DNA:
1. Form a small portion of the human genome
2. Code for proteins that are essential for survival
3. Code for proteins that are not essential for survival
4. Show a high degree of polymorphism
Subtopic: DNA Fingerprinting |
84%
From NCERT
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What will not be true for satellite DNA?
| 1. | Satellite DNA forms small peaks other than the bulk DNA during density gradient centrifugation. |
| 2. | Depending on the base composition (A: T rich or G: C rich), length of the segment, and the number of repetitive units, the satellite DNA is classified into many categories, such as micro-satellites, mini-satellites etc. |
| 3. | These sequences normally do not code for any proteins, but they form a large portion of the human genome. |
| 4. | These sequences do not show any polymorphism and form the basis of DNA fingerprinting. |
Subtopic: DNA Fingerprinting |
88%
From NCERT
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What will not be true regarding polymorphism in DNA?
| 1. | Polymorphism (variation at the genetic level) arises due to mutations. |
| 2. | Allelic sequence variation has traditionally been described as a DNA polymorphism if more than one variant (allele) at a locus occurs in the human population with a frequency greater than 1.00. |
| 3. | The probability of polymorphic variation to be observed in non-coding DNA sequence would be higher as mutations in these sequences may not have any immediate effect/impact on an individual’s reproductive ability. |
| 4. | For evolution and speciation, polymorphisms play a very important role. |
Subtopic: DNA Fingerprinting |
77%
From NCERT
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Consider the given two statements:
| Assertion (A): | DNA fingerprinting is the basis of paternity testing in cases of disputes. |
| Reason (R): | Polymorphism evaluated in DNA fingerprinting are inheritable from parents to children. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True and (R) does not correctly explain (A) |
| 3. | (A) is True, (R) is False |
| 4. | (A) is False, (R) is False |
Subtopic: DNA Fingerprinting |
92%
From NCERT
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Consider the given two statements:
| Assertion (A): | DNA fingerprinting is the basis of paternity testing, in case of disputes. |
| Reason (R): | DNA from every tissue (such as blood, hair-follicle, skin, bone, saliva, sperm etc.), from an individual show the same degree of polymorphism. |
| 1. | Both (A) and (R) are True but (R) does not correctly explain (A). |
| 2. | (A) is True but (R) is False. |
| 3. | (A) is False but (R) is True. |
| 4. | Both (A) and (R) are True and (R) correctly explains (A). |
Subtopic: DNA Fingerprinting |
54%
From NCERT
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The technique of DNA fingerprinting was developed initially by:
| 1. | Alfred Sturtevant | 2. | Alec Jeffrys |
| 3. | Thomas Cech | 4. | Fire and Mello |
Subtopic: DNA Fingerprinting |
93%
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Arrange the given steps in the process of DNA fingerprinting in correct chronological order [from earliest to last]:
| (i) | isolation of DNA |
| (ii) | separation of DNA fragments by electrophoresis |
| (iii) | digestion of DNA by restriction endonucleases |
| (iv) | hybridisation using labelled VNTR probe |
| (v) | detection of hybridised DNA fragments by autoradiography |
| (vi) | transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon |
| 1. | (i) – (ii) – (iii) – (vi) – (iv) – (v) |
| 2. | (i) – (iii) – (ii) – (vi) – (iv) – (v) |
| 3. | (ii) – (iii) – (i) – (vi) – (v) – (iv) |
| 4. | (iii) – (i) – (ii) – (v) – (iv) – (vi) |
Subtopic: DNA Fingerprinting |
84%
From NCERT
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Southern blotting is transfer to nitrocellulose sheet from agar gel of:
| 1. | RNA | 2. | Proteins |
| 3. | DNA | 4. | Lipids |
Subtopic: DNA Fingerprinting |
86%
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Transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization is called as:
| 1. | DNA profiling | 2. | PCR |
| 3. | Southern blotting | 4. | Elution |
Subtopic: DNA Fingerprinting |
75%
From NCERT
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