Which of the following is not a component of downstream processing?
1. Separation
2. Purification
3. Preservation
4. Expression
The Taq polymerase enzyme is obtained from
1. Thiobacillus ferroxidans
2. Bacillus subtilis
3. Pseudomonas subtilis
4. Thermus aquaticus
The cutting of DNA at specific locations became possible with the discovery of
(1) restriction enzymes
(2) probes
(3) selectable markets
(4) ligases
The DNA molecule to which the gene of interest is integrated for cloning is called:
1. | Transformer | 2. | Vector |
3. | Template | 4. | Carrier |
DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by
1. | centrifugation |
2. | polymerase chain reaction |
3. | electrophoresis |
4. | restriction mapping |
Which of the following is not correctly matched for the organism and its cell wall
degrading enzyme?
1. Bacteria —Lysozyme
2. Plant cells — Cellulase
3. Algae —Methylase
4. Fungi — Chitinase
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
1. | Non-recombinant bacteria containing -galactosidase |
2. | Insertional inactivation of -galactosidase in non-recombinant bacteria |
3. | Insertional inactivation of β-galactosidase in recombinant bacteria |
4. | Inactivation of glycosidase enzyme in recombinant bacteria |
Which one is a true statement regarding DNA polymerase used in PCR?
1. It is used to ligate introduced DNA in recipient cells
2. It serves as a selectable marker
3. It is isolated from a virus
4. It remains active at high temperature
For transformation, microparticles coated with DNA to be bombarded with gene gun are
made up of
1. silver or platinum
2. platinum or zinc
3. silicon or platinum
4. gold or tungsten
Given below is a sample of portion of DNA strand giving the base sequence on the
opposite strands. What is so, special shown in it?
5'__ GAATTC_ 3'
3' __ CTTAAG__5'
1. Deletion mutation
2. Start codon at the 5' end
3. Palindromic sequence of base pairs
4. Replication completed