- 1(current)
Q. No.Which of the following is not part of the normal process of cloning recombinant DNA in bacteria?
| 1. | restriction endonuclease digestion of cellular and plasmid DNAs. |
| 2. | production of recombinant DNA using DNA ligase and a mixture of digested cellular and plasmid DNAs. |
| 3. | separation of recombinant DNAs by electrophoresis using the Southern technique to determine where the desired recombinant migrates. |
| 4. | transformation of bacteria by the recombinant DNA plasmids and selection using ampicillin. |
Subtopic: Host & Desired DNA: II | Process of Biotech | Host & Desired DNA |
52%
Level 3: 35%-60%
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Identify the incorrect statement:
| 1. | The process of separation and purification of expressed protein before marketing is called as downstream processing |
| 2. | DNA precipitation out of a mixture of biomolecules can be achieved by treatment with a divalent cation such as calcium ion |
| 3. | Stirred-tank bioreactors have been designed for availability of oxygen throughout the process |
| 4. | Reporter genes can be used as markers for selecting successfully transformed cells |
Subtopic: Process of Biotech |
63%
Level 2: 60%+
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- 1(current)
Q. No.
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