1. | a nematode, Meloidogyne incognita. |
2. | a nematode, Caenorhabditis elegans. |
3. | a trematode, Clonorchis sinensis. |
4. | a cestode, Taenia sloium. |
RNAi, as a mechanism of cellular defense, takes place in:
1. | All bacteria | 2. | All unicellular organisms |
3. | All plants only | 4. | All eukaryotic organisms |
RNA interference involves silencing a specific mRNA by:
1. | interfering with its post transcriptional modification |
2. | not allowing the transport of mRNA from nucleus to cytoplasm |
3. | a dsRNA that binds to it and prevents its translation |
4. | post translational degradation of the protein |
Identify the incorrect statement regarding the use of RNAi for creating transgenic tobacco plants resistant to Meloidogyne incognita:
1. | Agrobacterium tumefaciens was used to transform the plant cells |
2. | Double-stranded RNA was introduced into the plant cells |
3. | Double-stranded RNA was formed in the plant cells that initiated RNAi |
4. | A specific mRNA of the nematode was silenced with the help of dsRNA |
I: | An infection by viruses having RNA genomes |
II: | Mobile genetic elements that replicate via an RNA intermediate |
III: | Integration of phage DNA into the main chromosomal DNA of a eukaryotic cell |
1. | Only I and II | 2. | Only I and III |
3. | Only II and III | 4. | I, II and III |
A biological process in which RNA molecules inhibit gene expression or translation, by neutralizing targeted mRNA molecules is known as:
1. | Bioprospecting | 2. | Molecular pharming |
3. | RNA interference | 4. | Insertional mutagenesis |
I: | involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA (silencing). |
II: | has been used to create transgenic tobacco plants resistant to nematode Meloidogyne incognita |