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Q. No.30 cycles of a polymerase chain reaction can increase the number of a DNA fragments to about:
1. 1,000,000
2. 10,000,000
3. 50,000,000
4. 1,000,000,000
Subtopic: Polymerase Chain Reaction: PCR |
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What is the purpose of using agarose gel electrophoresis in biotechnology processes?
| 1. | To replicate DNA fragments rapidly. |
| 2. | To cut DNA into smaller fragments using restriction enzymes. |
| 3. | To separate DNA fragments based on their size. |
| 4. | To join fragments of DNA from different sources. |
Subtopic: Separation and Isolation of DNA fragments |
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The importance of polymerase chain reaction (PCR) in biotechnology lies in its ability to:
| 1. | Quickly synthesize small DNA fragments for gene therapy. |
| 2. | Amplify large quantities of DNA from a small sample. |
| 3. | Cut specific DNA fragments for cloning. |
| 4. | Directly insert genes into living cells. |
Subtopic: Polymerase Chain Reaction: PCR |
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The requirement of primers in a polymerase chain reaction (PCR) is because they:
| 1. | Provide the starting point for DNA synthesis. |
| 2. | Help in cutting the DNA into smaller fragments. |
| 3. | Join the DNA fragments after amplification. |
| 4. | Act as selectable markers. |
Subtopic: Polymerase Chain Reaction: PCR |
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Isolation of DNA in pure form from an animal cell involves:
| 1. | Adding alcohol to precipitate the DNA. |
| 2. | Heating the cell to denature DNA. |
| 3. | Using enzymes to cut the DNA into smaller fragments. |
| 4. | Exposing the cell to ultraviolet light. |
Subtopic: Separation and Isolation of DNA fragments |
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Consider the given two statements:
| Assertion (A): | Downstream processing is vital in biotechnology for the purification of products. |
| Reason (R): | It ensures that the products are of high purity and quality, suitable for industrial and clinical use. |
| 1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
| 2. | Both (A) and (R) are True but (R) is not the correct explanation of (A) |
| 3. | (A) is True but (R) is False |
| 4. | (A) is False but (R) is True |
Subtopic: Bioreactors/Downstream Processing |
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Consider the two statements:
| Assertion (A): | A continuous culture is likely to produce more biomass in industrial production of recombinant products |
| Reason (R): | In continuous culture, the microbes are continuously kept in their log phase of growth. |
| 1. | Both (A) and (R) are True and (R) correctly explains (A) |
| 2. | Both (A) and (R) are True but (R) does not correctly explain (A) |
| 3. | (A) is True, (R) is False |
| 4. | (A) is False, (R) is True |
Subtopic: Bioreactors/Downstream Processing |
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Match the enzyme with its function in genetic engineering.
1. A-2, B-3, C-1, D-4
2. A-2, B-1, C-3, D-4
3. A-3, B-1, C-2, D-4
4. A-4, B-3, C-1, D-2
| Column I | Column II | ||
| A. | DNA polymerase | 1. | Joining DNA fragments |
| B. | Restriction endonuclease | 2. | Synthesizes new strands of DNA |
| C. | DNA ligase | 3. | Cuts DNA at specific sequences |
| D. | Reverse transcriptase | 4. | Synthesizes DNA from RNA template |
1. A-2, B-3, C-1, D-4
2. A-2, B-1, C-3, D-4
3. A-3, B-1, C-2, D-4
4. A-4, B-3, C-1, D-2
Subtopic: Restriction Enzymes - Main Enzymes |
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Pfu DNA polymerase is used in:
| 1. | amplifying DNA in the polymerase chain reaction (PCR). |
| 2. | creation of blunt ends in digested DNA fragments. |
| 3. | artificial synthesis of DNA in a template independent manner. |
| 4. | proof reading of newly synthesized DNA strands. |
Subtopic: Polymerase Chain Reaction: PCR |
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Who was awarded Nobel prize for the invention of PCR?
| 1. | Thomas Cech | 2. | Fred Sanger |
| 3. | Kary Mullis | 4. | Millstein and Kohler |
Subtopic: Polymerase Chain Reaction: PCR |
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