| List-I | List-II | ||
| (A) | Gene therapy | (I) | Separation of DNA fragments |
| (B) | RNA interference | (II) | Diagnostic test for AIDS |
| (C) | ELISA | (III) | Cellular defense |
| (D) | Gel Electrophoresis | (IV) | Allows correction of a gene defect |
| Options: | (A) | (B) | (C) | (D) |
| 1. | IV | I | II | III |
| 2. | IV | II | III | I |
| 3. | IV | III | II | I |
| 4. | IV | III | I | II |
The first human hormone produced by recombinant DNA technology is:
1. Insulin
2. Estrogen
3. Thyroxin
4. Progesterone
1. Escherichia
2. Mycobacterium
3. Rhizobium
4. Saccharomyces
Which one of the following is now being commercially produced by biotechnological procedures:
1. Morphine
2. Quinine
3. Insulin
4. Nicotine
| Statement I: | Two DNA sequences were prepared corresponding to A and B, chains of human insulin and were introduced in the plasmids of Agrobacterium to produce insulin chain. |
| Statement II: | Chain A and B were produced separately, extracted and combined by creating disulphide bonds to form human insulin. |
| 1. | Mature insulin molecule consists of three polypeptide chains - A, B and C. |
| 2. | Insulin is synthesized as prohormone which contains extra stretch of C-peptide. |
| 3. | C-peptide is not present in mature insulin molecule. |
| 4. | Polypeptide chains A and B are linked to disulphide bridges. |
| (a) | Pro-hormone insulin contain extra stretch of C-peptide |
| (b) | A-peptide and B-peptide chains of insulin were produced separately in E.coli, extracted and combined by creating disulphide bond between them. |
| (c) | Insulin used for treating Diabetes was extracted from Cattles and Pigs. |
| (d) | Pro-hormone Insulin needs to be processed for converting into a mature and functional hormone. |
| (e) | Some patients develop allergic reactions to the foreign insulin. |
| 1. | (c), (d) and (e) only | 2. | (a), (b) and (d) only |
| 3. | (b) only | 4. | (c) and (d) only |
Genetically engineered insulin for human is produced from:
1. Escherichia coli
2. Psuedomonas putida
3. Bacillus thuringiensis
4. Rhizobium meliloti
Give below are two statements : one is labelled as Assertion (A) and the other is labelled as Reason (R).
| Assertion (A): | In human beings, insulin is synthesized as a pro-hormone which needs to be processed before it becomes fully mature and functional. |
| Reason (R): | The extra stretch of C-peptide is to be removed from A and B peptide chain of insulin. |
In the light of the above statements, Choose the most appropriate answer from the option given below :
| 1. | (A) is True but (R) is False. |
| 2. | (A) is False but (R) is True. |
| 3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
| 4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
With regard to insulin, choose the correct options:
| (a) | C-peptide is not present in mature insulin. |
| (b) | The insulin produced by rDNA technology has C-peptide. |
| (c) | The pro-insulin has C-peptide. |
| (d) | A-peptide and B-peptide of insulin are interconnected by disulphide bridges. |
| 1. | (a), (c) and (d) only | 2. | (a) and (d) Only |
| 3. | (b) and (d) only | 4. | (b) and (c) only |