Consider the following statements:
I. | Ori is responsible for copy number of the linked DNA. |
II. | Transformation is the process through which a piece of DNA is introduced into a host bacterium. |
III. | Bacteriophages have very high copy numbers of their genome within the bacterial cell. |
Which of the above statements are true?
1. I and II only
2. I and III only
3. II and III only
4. I, II and III
Column I | Column II | ||
A. | Lysozyme | P. | Fungal cell |
B. | Cellulase | Q. | Plant cell |
C. | Chitinase | R. | Bacterial cell |
A | B | C | |
1. | P | Q | R |
2. | P | R | Q |
3. | R | P | Q |
4. | R | Q | P |
I: | Agar Gel electrophoresis is useful in analysis of DNA. |
II: | DNA is generally positively charged due to its nitrogenous bases so an electrical field forces it to move toward a negative pole. |
1. | Both I and II are correct and II explains I |
2. | Both I and II are correct but II does not explain I |
3. | I is correct but II is incorrect |
4. | Both I and II are incorrect |
I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
1. | Both I and II are correct and II explains I |
2. | Both I and II are correct but II does not explain I |
3. | I is correct but II is incorrect |
4. | Both I and II are incorrect |
Assertion (A): | Thermostable DNA dependent DNA polymerase is used in Polymerase Chain Reaction |
Reason (R): | Such enzymes do not require primers and can initiate DNA synthesis |
1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A) |
3. | (A) is True but (R) is False |
4. | Both (A) and (R) are False |
Assertion (A): | In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. |
Reason (R): | Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. |
1. | Both (A) and (R) are True and the (R) is the correct explanation of (A) |
2. | Both (A) and (R) are True but the (R) is not the correct explanation of (A) |
3. | (A) is True but (R) is False |
4. | Both (A) and (R) are False |
Assertion (A): | When constructing rDNA, the vector DNA should never be cut by the same restriction endonuclease that was used to cut the donor DNA |
Reason (R): | Use of same restriction endonuclease will generate same kind of sticky ends retarding the function of DNA ligase. |
1. | Both (A) and (R) are True and (R) is the correct explanation of (A) |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A) |
3. | (A) is True but (R) is False |
4. | Both (A) and (R) are False |
Assertion (A): | Polymerase chain reaction is used in DNA amplification. |
Reason (R): | The ampicillin resistant gene is used as a selectable marker to check transformation. |
1. | (A) is not correct but (R) is correct. |
2. | Both (A) and (R) are correct and (R) is the correct explanation of (A). |
3. | Both (A) and (R) are correct but (R) is not the correct explanation of (A). |
4. | (A) is correct but (R) is not correct. |
Statement I: | Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. |
Statement II: | Restriction endonucleases cut the DNA strand a little away from the centre of the Palindromic site. |
Given below are two statements: One is labelled as Assertion (A) and the other is labelled as Reason (R)
Assertion (A): | Restriction enzyme is a type of endonuclease |
Reason (R): | Restriction enzyme cuts the two stands of DNA at specific positions within the DNA |
In the light of the above statements, choose the correct answer from the options given below:
1. | (A) is True but (R) is False. |
2. | (A) is False but (R) is True. |
3. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
4. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |