I: | Plasmid can be used as a vector in recombinant DNA technology experiments. |
II: | Plasmid is an extrachromosomal, circular, double-stranded DNA found in some bacteria. |
1. | Both I and II are correct and II explains I |
2. | Both I and II are correct but II does not explain I |
3. | I is correct but II is incorrect |
4. | Both I and II are incorrect |
Identify the properties of a good vector used in rDNA technology:
(a) | It should have origin of replication supporting a high copy number |
(b) | It should have preferably more than '2' recognition sites |
(c) | The restriction sites in vector should be in the antibiotic- resistant genes |
(d) | It should have suitable marker genes |
(e) | It should be easy to isolate and purify |
Choose the most appropriate answer from the options given below:
1. (a), (c) and (e) onlyStatement A: | Bacteriophages have very high copy numbers of their genome within the bacterial cells |
Statement B: | To recover many copies of the target DNA, it should not be cloned in a vector whose origin supports high copy number |
Assertion(A): | Plasmids as cloning vectors usually get integrated to the host cells chromosome after transformation. |
Reason(R): | Plasmids get disintegrated after transformation. |
1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
3. | (A) is True but (R) is False. |
4. | Both (A) and (R) are False. |
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