Given below are a few statements regarding somatic hybridization. Choose the correct statements.
I. | Protoplasts of different cells of the same plant are fused. |
II. | Protoplasts from cells of different species can be fused. |
III. | Treatment of cells with cellulase and pectinase is mandatory. |
IV. | The hybrid protoplast contains characters of only one parental protoplast. |
1. I and III
2. I and II
3. I and IV
4. II and III
Statement I: | Proteins encoded by genes cryIAc and cryllAb control corn borer, that of crylAb control the cotton bollworms |
Statement II: | Bt toxin protein exist as inactive protoxins but once an insect ingests the inactive toxin, it is converted into an active form of toxin due to the alkaline pH of the gut |
Statement I: | Bacillus thuringiensis forms protein crystals which contain a toxin that kills certain insects but not Bacillus because Bt toxin protein exists as inactive protoxins in Bacillus. |
Statement II: | Bacillus thuringiensis forms protein crystals which contain a toxin that kills certain insects but not Bacillus because toxin requires alkaline pH available in the gut of insects and which solubilises the crystals in order to convert into an active form. |
(i) | PCR is used to detect mutations in genes in suspected cancer patients. |
(ii) | A direct method to insert a foreign gene into plant cells is micro-injection. |
(iii) | The first transgenic cow, Rosie, produced human protein-enriched milk, i.e., 5.4 grams per litre. |
(iv) | RNAi takes place in all eukaryotic organisms as a method of cellular defense. |
Column I | Column II | ||
a. | Patent on Basmati rice by an American company | (i) | 1983 |
b. | First clinical gene therapy | (ii) | 1997 |
c. | Humulin by Eli Lilly Co. | (iii) | 1972 |
d. | Construction of first recombinant DNA | (iv) | 1990 |
a. | C-peptide is found attached with both A and B chains of insulin with the help of disulphide bonds. |
b. | In 1983, both A and B chains were produced by introducing alien genes in plasmid of same E.coli by Eli Lilly. |
c. | A-peptide and B-peptide of insulin are interconnected by peptide bonds in mature insulin. |
d. | Insulin from an animal source may cause allergy or other types of reactions in some diabetic patients. |
Assertion (A): | Insulin used for diabetes was earlier extracted from the pancreas of slaughtered cattle and pigs but now it is not used. |
Reason (R): | Aarti along with some other patients took insulin injections in which insulin was from an animal source and they manifested an allergic response to that. |
1. | Both (A) and (R) are True and (R) is the correct explanation of (A). |
2. | Both (A) and (R) are True but (R) is not the correct explanation of (A). |
3. | (A) is True but (R) is False. |
4. | (A) is False but (R) is True. |